pe conjugated rat anti mouse ulbp  (R&D Systems)

Journal: PLoS ONE

Article Title: Dendritic Cell Based PSMA Immunotherapy for Prostate Cancer Using a CD40-Targeted Adenovirus Vector

doi: 10.1371/journal.pone.0046981

Figure Lengend Snippet: (A) Cytotoxic activity of NK effector cells on YAC-1 and RM-1 target cells was determined by a 51 Cr-release assay at indicated E:T ratios. Target cells used were YAC-1 (•), RM-1 parental (▾), RM-1 parental cells pre-treated by infection with Ad5-IFNγ (▪), RM-1-PSMA clone 3 cells (⧫), or RM-1-PSMA clone 3 cells pre-treated by infection with Ad5-IFNγ (▴). Each data point represents the mean ± standard error of four replicate wells. (B) Flow cytometry analysis of YAC-1 and RM-1 cells stained with PE labeled anti-H60, anti-MULT-1, or anti-Rae-1 antibodies or with an isotype-matched control antibody. Numbers below the histograms correspond to mean fluorescence intensity of each peak.

Article Snippet: The cells were incubated for 1 h at 4°C with phycoerythrin (PE) labeled rat IgG 2A isotype control antibody (R&D Systems, Minneapolis, MN) or with the following antibodies: (a) a PE labeled rat anti-mouse H60 monoclonal antibody (R&D Systems), (b) a PE labeled rat anti-mouse Rae-1 (pan-specific) monoclonal antibody (R&D Systems), or (c) a PE labeled rat anti-mouse MULT-1 monoclonal antibody (R&D Systems).

Techniques: Activity Assay, Release Assay, Infection, Flow Cytometry, Staining, Labeling, Fluorescence